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Safety and tolerability of topically administered autologous, apoptotic PBMC secretome (APOSEC) in dermal wounds: a randomized Phase 1 trial (MARSYAS I).
Developing effective therapies against chronic wound healing deficiencies is a global priority. Thus we evaluated the safety of two different doses of topically administered autologous APOSEC, the secretome of apoptotic peripheral blood mononuclear cells (PBMCs), in healthy male volunteers with artificial dermal wounds. Ten healthy men were enrolled in a single-center, randomized, double-blinded, placebo-controlled phase …
Towards clinically friendly optoretinography (ORG) using 100 kHz swept-source OCT without adaptive optics (AO)
Optoretinography measures the stimulus-evoked response of the photoreceptors using light. Current systems use adaptive optics (AO) to obtain cellular-level resolution and fast volume acquisitions, to be able to monitor a single cone’s response with sufficient sampling over time. The systems are complex to operate and therefore are not ideal in the clinical setting, where ease-of-use …
Nocardiosis in the Illawarra-Shoalhaven region from 2010 to 2019.
There is a need for simple microbiology diagnostics to enable antimicrobial resistance surveillance in low- and middle-income countries. To investigate the field utility of InTray COLOREX plates for urine culture and ESBL detection. Clinical urine samples from Mahosot Hospital, Vientiane, Lao PDR were inoculated onto chromogenic media and InTray COLOREX Screen plates between June and August 2020. …
Nocardiosis in the Illawarra-Shoalhaven region from 2010 to 2019. Read More »
Serum-free media supplements carry miRNAs that co-purify with extracellular vesicles.
Recent studies on extracellular RNA raised awareness that extracellular vesicles (EVs) isolated from cultured cells may co-purify RNAs derived from media supplements such as fetal bovine serum (FBS) confounding EV-associated RNA. Defined culture media supplemented with a range of nutrient components provide an alternative to FBS addition and allow EV-collection under full medium conditions avoiding …
Serum-free media supplements carry miRNAs that co-purify with extracellular vesicles. Read More »
Circulating Tumor DNA Using Tagged Targeted Deep Sequencing to Assess Minimal Residual Disease in Breast Cancer Patients Undergoing Neoadjuvant Chemotherapy.
In breast cancer patients undergoing neoadjuvant chemotherapy before surgery, there is an unmet need for non-invasive biomarkers predictive of response. Analysis of DNA circulating tumor (ctDNA) in particular has been the object of several reports, but some of them have studied the application of targeted sequencing marked in (tTDS) for clinical practice and performance compared …
Genetic diversity, population structure, and relationships of apricot (Prunus) based on restriction site-associated DNA sequencing.
Single-nucleotide polymorphisms (SNPs) are the most abundant form of genomic polymorphism and is widely used in population genetics research. Here, the high-throughput sequencing is used to test the genome-level diversity, population structure, and relationships apricots, which are important for the conservation of germplasm and molecular breeding. Restriction-site associated DNA sequencing (RAD-seq) adopted to sequence 168 …