Analysis of DNA methylation profiles during sheep skeletal muscle development using whole-genome bisulfite sequencing.

In breast cancer patients undergoing neoadjuvant chemotherapy before surgery, there is an unmet need for non-invasive biomarkers predictive of response. Analysis of DNA circulating tumor (ctDNA) in particular has been the object of several reports, but some of them have studied the application of targeted sequencing marked in (tTDS) for clinical practice and performance compared to digital droplet PCR (ddPCR). Here, we present the first results of an ongoing study involving prospectively accrued, monocentric cohort patients with invasive breast cancer, underwent neoadjuvant chemotherapy followed by surgery with curative intent as per clinical practice.

A pretreatment tumor biopsies and plasma samples were collected before and during treatment, after surgery, and every six months thereafter or until relapse, whichever comes first. pretreatment biopsies were sequenced with 409-gene panel large parallel sequencing (MPS), which enable the identification of mutations in the target and their research in plasma by tTDS and ddPCR as complementary approaches. Using tTDS, we showed the presence of at least one deleterious mutations in all cases of relapse we study (n = 4), with an average lead time of six months before clinical relapse. Association with ddPCR is suboptimal, and only one patient relapse can be identified by the method. tTDS shows potential as a non-invasive method for the early detection of MRD in patients with SM.


Next-generation sequencing (NGS) an interesting alternative approach to PCR-based characterization of plant genetic engineering for security assessment and labeling since NGS is highly sensitive in detecting insertion of T-DNA and vector backbone sequences in transgenic plants. In this study, two transgenic lines independent male Populus tremula, T193-2 and T195-1, both of which carry the gene FLOWERING LOCUS T of Arabidopsis thaliana under the control of the heat-inducible promoter (pHSP :: AtFT) and non-transgenic control W52 clone, which is further characterized by NGS and third generation sequencing.

The results support previous findings that T-DNA hemizygously included in the genomic loci of each line. However, T-DNA insertion consists of a conglomeration of one or two copies of the T-DNA together with T-DNA fragments smaller without AtFT section. Based on data from NGS, no extra pieces of T-DNA or vector backbone sequences can be identified in the genome of two transgenic lines. Therefore, the seeds derived from a cross between pHSP :: AtFT elderly male and female transgenic wild plants are expected T-DNA vector backbone fragment or free. Thus, PCR analysis of strengthening the T-DNA fragment with a primer AtFT partial specific enough to determine whether or not the transgenic seeds.

Western Blot Blocker

20960007-1 250 g
EUR 32.46

Western Blot Blocker

20960007-2 500 g
EUR 60.54

Western Blot Marker

TTOPP-2301 20Lanes
EUR 310.44

SSB Western Blot Kit

GWB-4759BD Kit Ask for price

ERG Western Blot Kit

GWB-589385 Kit Ask for price

ID4 Western Blot Kit

GWB-232633 Kit Ask for price

FST Western Blot Kit

GWB-31B3A5 Kit Ask for price

EED Western Blot Kit

GWB-31EF96 Kit Ask for price

AHR Western Blot Kit

GWB-EFD465 Kit Ask for price

OTC Western Blot Kit

GWB-CED6C6 Kit Ask for price

ENG Western Blot Kit

GWB-C2F17D Kit Ask for price

ASPN Western Blot Kit

GWB-3F3BF8 Kit Ask for price

SPP1 Western Blot Kit

GWB-4172CB Kit Ask for price

ETS1 Western Blot Kit

GWB-419F49 Kit Ask for price

G3BP Western Blot Kit

GWB-46B393 Kit Ask for price

BRD7 Western Blot Kit

GWB-51BDA0 Kit Ask for price

ZBP1 Western Blot Kit

GWB-5E94FB Kit Ask for price

MSI2 Western Blot Kit

GWB-24AE67 Kit Ask for price

GLI2 Western Blot Kit

GWB-2A323E Kit Ask for price

TSHR Western Blot Kit

GWB-2D5460 Kit Ask for price

RNF2 Western Blot Kit

GWB-31E776 Kit Ask for price

ILF2 Western Blot Kit

GWB-1149E0 Kit Ask for price

LHX6 Western Blot Kit

GWB-13F4C0 Kit Ask for price

TPM1 Western Blot Kit

GWB-16A472 Kit Ask for price

GLI1 Western Blot Kit

GWB-888E77 Kit Ask for price

SOX5 Western Blot Kit

GWB-908D84 Kit Ask for price

NPM1 Western Blot Kit

GWB-AA50AA Kit Ask for price

A1BG Western Blot Kit

GWB-FA7CD7 Kit Ask for price

LIG4 Western Blot Kit

GWB-FAE800 Kit Ask for price

PAX7 Western Blot Kit

GWB-DF90D0 Kit Ask for price

PAX7 Western Blot Kit

GWB-EEA80D Kit Ask for price

RORA Western Blot Kit

GWB-DC713B Kit Ask for price

PDCD4 Western Blot Kit

GWB-37F683 Kit Ask for price

TNRC4 Western Blot Kit

GWB-3AECE0 Kit Ask for price

GNB1L Western Blot Kit

GWB-3C89D6 Kit Ask for price

PITX2 Western Blot Kit

GWB-3D38F8 Kit Ask for price

RPS16 Western Blot Kit

GWB-407949 Kit Ask for price

STK11 Western Blot Kit

GWB-4A089E Kit Ask for price

PTPN2 Western Blot Kit

GWB-4A1E76 Kit Ask for price

IDH3A Western Blot Kit

GWB-5ED2E6 Kit Ask for price

STAU1 Western Blot Kit

GWB-236BD7 Kit Ask for price

GABRP Western Blot Kit

GWB-24C55E Kit Ask for price

TRPM3 Western Blot Kit

GWB-28C4BA Kit Ask for price

HIPK2 Western Blot Kit

GWB-2CAB70 Kit Ask for price

ESRRG Western Blot Kit

GWB-306860 Kit Ask for price

WNT2B Western Blot Kit

GWB-31DC35 Kit Ask for price

ANXA1 Western Blot Kit

GWB-11A960 Kit Ask for price

SATB1 Western Blot Kit

GWB-1505B2 Kit Ask for price

RSAD2 Western Blot Kit

GWB-158EF5 Kit Ask for price

HTR1A Western Blot Kit

GWB-843597 Kit Ask for price

P2RX7 Western Blot Kit

GWB-8C5BF1 Kit Ask for price

KCNN2 Western Blot Kit

GWB-8E971E Kit Ask for price

SOX10 Western Blot Kit

GWB-93F38B Kit Ask for price

ZFP67 Western Blot Kit

GWB-9EC907 Kit Ask for price

OLIG2 Western Blot Kit

GWB-6EC267 Kit Ask for price

PCBP1 Western Blot Kit

GWB-7D2B23 Kit Ask for price

PANX2 Western Blot Kit

GWB-F284D9 Kit Ask for price

CLCN3 Western Blot Kit

GWB-F33E4A Kit Ask for price

TGFBI Western Blot Kit

GWB-F84989 Kit Ask for price

PANX1 Western Blot Kit

GWB-FD36EC Kit Ask for price

RUNX2 Western Blot Kit

GWB-FFA7E7 Kit Ask for price

ZFP36 Western Blot Kit

GWB-B1BCFA Kit Ask for price

PBEF1 Western Blot Kit

GWB-EA0DE7 Kit Ask for price

RAB14 Western Blot Kit

GWB-EF804C Kit Ask for price

ZFP42 Western Blot Kit

GWB-CABDE0 Kit Ask for price

KEAP1 Western Blot Kit

GWB-CD9C1D Kit Ask for price

ATP7A Western Blot Kit

GWB-D12842 Kit Ask for price

JMJD3 Western Blot Kit

GWB-D8EFB3 Kit Ask for price

CLIC1 Western Blot Kit

GWB-DA38C2 Kit Ask for price

ARIH2 Western Blot Kit

GWB-DB4558 Kit Ask for price

KRT13 Western Blot Kit

GWB-C3423B Kit Ask for price

TARDBP Western Blot Kit

GWB-3E00CF Kit Ask for price

HNRPA3 Western Blot Kit

GWB-1EAFD5 Kit Ask for price

SNRP70 Western Blot Kit

GWB-12C365 Kit Ask for price

TWIST1 Western Blot Kit

GWB-172C7E Kit Ask for price

HNRPLL Western Blot Kit

GWB-72079F Kit Ask for price

SLC1A5 Western Blot Kit

GWB-F244A5 Kit Ask for price

TARDBP Western Blot Kit

GWB-DC48CE Kit Ask for price

RHOBTB1 Western Blot Kit

GWB-16F5AC Kit Ask for price

SLC22A7 Western Blot Kit

GWB-A3D4FB Kit Ask for price

SLC12A1 Western Blot Kit

GWB-F10DDC Kit Ask for price

ONECUT2 Western Blot Kit

GWB-D5E58E Kit Ask for price

SLC22A1 Western Blot Kit

GWB-D6EAC0 Kit Ask for price

CEACAM6 Western Blot Kit

GWB-DA1310 Kit Ask for price

Rat Placenta Western Blot

RW-413 1 Blot
EUR 668

Western Blot Enhancing Kit

K3172120 1200 cm2
EUR 123

Western Blot Enhancing Kit

K3172250 2500 cm2
EUR 182

Western Blot Detection Kit

E-IR-R304A-50Assays 50Assays
EUR 210

Western Blot Detection Kit

E-IR-R304A-each each Ask for price

cyclin G2 Western Blot Kit

GWB-127000 Kit Ask for price

Skin Western Blot, 14 species

AW-101 1 Blot
EUR 1031

Lung Western Blot, 14 species

AW-601 1 Blot
EUR 1031

Brain Western Blot, 14 species

AW-201 1 Blot
EUR 1031

Colon Western Blot, 14 species

AW-311 1 Blot
EUR 1031

Liver Western Blot, 14 species

AW-314 1 Blot
EUR 1031

Ovary Western Blot, 14 species

AW-406 1 Blot
EUR 1031

Heart Western Blot, 14 species

AW-801 1 Blot
EUR 1031

Rat Whole Embryo Western Blot

RW-104 1 Blot
EUR 668

Testis Western Blot, 14 species

AW-401 1 Blot
EUR 1031

Kidney Western Blot, 14 species

AW-901 1 Blot
EUR 1031

AHR Western Blot kit (AWBK32243)

AWBK32243 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ENG Western Blot kit (AWBK33068)

AWBK33068 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ID4 Western Blot kit (AWBK38058)

AWBK38058 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

EED Western Blot kit (AWBK38384)

AWBK38384 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SSB Western Blot kit (AWBK40462)

AWBK40462 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

OTC Western Blot kit (AWBK41766)

AWBK41766 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

FST Western Blot kit (AWBK42287)

AWBK42287 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TMB Substrate, for Western Blot

F100029 1000 ml
EUR 992.4
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

TMB Substrate, for Western Blot

F129-100 100 ml
EUR 298.8
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

Rat Mammary Gland Western Blot

RW-414 1 Blot
EUR 668

Augurin (Human) - Western Blot Kit

WBK-012-25 1 kit
EUR 822.96

Ghrelin (Human) - Western Blot Kit

WBK-031-30 1 kit
EUR 822.96

Dog Tissue Premade Western Blot

DW-MT-1 1 Blot
EUR 1031

Muscles Western Blot, 14 species

AW-102 1 Blot
EUR 1031

Stomach Western Blot, 14 species

AW-302 1 Blot
EUR 1031

ERG Western Blot kit (AWBK100625)

AWBK100625 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

GLI2 Western Blot kit (AWBK31885)

AWBK31885 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PAX4 Western Blot kit (AWBK32064)

AWBK32064 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

GLI1 Western Blot kit (AWBK32368)

AWBK32368 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PAX7 Western Blot kit (AWBK32393)

AWBK32393 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

LHX6 Western Blot kit (AWBK32553)

AWBK32553 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PAX7 Western Blot kit (AWBK32742)

AWBK32742 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SOX5 Western Blot kit (AWBK33323)

AWBK33323 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

A1BG Western Blot kit (AWBK33810)

AWBK33810 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity: Human
  • Application:
  • Assay info:
  • Sensitivity:

NPM1 Western Blot kit (AWBK34094)

AWBK34094 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

LIG4 Western Blot kit (AWBK34122)

AWBK34122 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RNF2 Western Blot kit (AWBK34290)

AWBK34290 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ILF2 Western Blot kit (AWBK35731)

AWBK35731 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SPP1 Western Blot kit (AWBK36677)

AWBK36677 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ZBP1 Western Blot kit (AWBK36711)

AWBK36711 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

G3BP Western Blot kit (AWBK37713)

AWBK37713 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

BRD7 Western Blot kit (AWBK39018)

AWBK39018 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

MSI2 Western Blot kit (AWBK41112)

AWBK41112 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TPM1 Western Blot kit (AWBK41392)

AWBK41392 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TSHR Western Blot kit (AWBK41870)

AWBK41870 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ASPN Western Blot kit (AWBK42487)

AWBK42487 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RORA Western Blot kit (AWBK45608)

AWBK45608 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

Mouse CD1 Placenta Western Blot

MW-413 1 Blot
EUR 668

Mouse C57 Placenta Western Blot

MW-413-C57 1 Blot
EUR 789

Pig Tissue Premade Western Blot

PW-MT-1 1 Blot
EUR 910

Pig Tissue Premade Western Blot

PW-MT-1-1 0.1mg
EUR 910

PDCD4 Western Blot kit (AWBK09045)

AWBK09045 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ETS1 Western Blot kit (AWBK100604)

AWBK100604 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

GABRP Western Blot kit (AWBK13034)

AWBK13034 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

HTR1A Western Blot kit (AWBK13041)

AWBK13041 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RAB14 Western Blot kit (AWBK13107)

AWBK13107 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TNRC4 Western Blot kit (AWBK31525)

AWBK31525 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ESRRG Western Blot kit (AWBK31655)

AWBK31655 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

HIPK2 Western Blot kit (AWBK32586)

AWBK32586 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ZFP67 Western Blot kit (AWBK32749)

AWBK32749 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

OLIG2 Western Blot kit (AWBK32753)

AWBK32753 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SOX10 Western Blot kit (AWBK33326)

AWBK33326 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RPS16 Western Blot kit (AWBK33535)

AWBK33535 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ATP7A Western Blot kit (AWBK33797)

AWBK33797 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ZFP36 Western Blot kit (AWBK34385)

AWBK34385 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ARIH2 Western Blot kit (AWBK34418)

AWBK34418 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

KEAP1 Western Blot kit (AWBK34728)

AWBK34728 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

CLIC1 Western Blot kit (AWBK35045)

AWBK35045 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

KCNN2 Western Blot kit (AWBK35094)

AWBK35094 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

CLCN3 Western Blot kit (AWBK35504)

AWBK35504 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

P2RX7 Western Blot kit (AWBK35518)

AWBK35518 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TRPM3 Western Blot kit (AWBK35609)

AWBK35609 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PITX2 Western Blot kit (AWBK35634)

AWBK35634 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ZFP42 Western Blot kit (AWBK36244)

AWBK36244 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ANXA1 Western Blot kit (AWBK36568)

AWBK36568 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RUNX2 Western Blot kit (AWBK36678)

AWBK36678 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

Bapx1 Western Blot kit (AWBK36826)

AWBK36826 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SATB1 Western Blot kit (AWBK38208)

AWBK38208 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

JMJD3 Western Blot kit (AWBK40102)

AWBK40102 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

STAU1 Western Blot kit (AWBK40537)

AWBK40537 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PCBP1 Western Blot kit (AWBK40630)

AWBK40630 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

WNT2B Western Blot kit (AWBK41254)

AWBK41254 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

GNB1L Western Blot kit (AWBK41328)

AWBK41328 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
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