Combined bacterial and fungal targeted amplicon sequencing of respiratory samples: Does the DNA extraction method matter?

High-throughput sequencing techniques were used to analyze the diversity of respiratory microbiota in health and disease. Despite the extensive data available on the respiratory tract bacterial microbiota, the remnants of the less studied fungal components. This is partly due to the technical problems associated with fungal metagenomics analysis. In this study, we compared two protocols of DNA extraction and amplification of two targets for the combined fungal bacteria and fungi that are targeted amplicon sequencing analysis of sputum microbiota.Six breathing, selected randomly from a sample routine Mondor Hospital (Créteil, France) and treated anonymously, were tested after a routine culture of bacteria and fungi.

Two of spiked Fumigati Aspergillus and Aspergillus Nigri (105 conidia / ml). After the mechanical lysis, DNA was extracted using the automatic extraction of QIAsymphony® (AQE) or users PowerSoil® MoBio extraction (MPE). DNA yield and purity compared. DNA extracted from sputum spiked into the target (i) real-time PCR for detection of Aspergillus DNA and (ii) a combination of primary targeting barcode metagenomic analysis for ITS1 and ITS2 fungi, and bacteria V1-V2 and V3-V4 region of the 16S. amplicon libraries were prepared using reagents MiSeq V3 kit on the Illumina platform. Data were analyzed using software PyroMIC © and Shaman, and compared with culture results.AQE extraction provides a higher yield of the (ratio AQE / MPE DNA = 4.5 [1.3 to 11]) DNA in a shorter period of time.

Results of Aspergillus DNA is detected by qPCR were similar to sputum spiked regardless of the extraction protocol. extraction quite an impact on biodiversity or the relative abundance of bacterial communities using targeted amplicon sequencing (2/43 taxa impact). For fungi, the relative abundance of major taxa 4/11 affected and AQE results were closer to the culture results. V1-V2 or V3-V4 and ITS1 or ITS2 target is judged diversity major taxa of bacteria and fungi, but ITS2 and V3-V4 detected smaller taxa.Our results show the importance of joint extraction of DNA for bacteria and fungi targeted metagenomics respiratory samples. Extraction protocol may affect the results of the DNA and the relative abundance of a few bacteria, but more fungal taxa. For the analysis of fungi, ITS2 allow detection of a large number of taxa small compared with ITS1.

ACTG1 human mutation associated with high frequency hearing loss, and patients with mutations in this gene are good candidates for the electric acoustic stimulation. To better understand the genetic etiology of hearing loss cases, massively parallel DNA sequencing performed on a 7048 Japanese probands related hearing loss. Among the 1,336 patients with autosomal dominant hearing loss, we identified 15 probands (1.1%) with 13 ACTG1 potentially pathogenic variant. Six new variant and seven were reported previously.

We collect and analyze detailed clinical features of these patients. The average level of development of pure-tone hearing worsening of the average for the four frequency is 1.7 dB / year of age 0-50 years, and all individuals over 60 years old has a severe hearing loss. To better understand the mechanisms underlying causes of disease, the intracellular localization of the wild-type and mutant gamma-actins checked using NIH / 3T3 fibroblast cell line

Western Blot Enhancing Kit

K3172120 1200 cm2
EUR 107

Western Blot Enhancing Kit

K3172250 2500 cm2
EUR 158

AHR Western Blot kit (AWBK32243)

AWBK32243 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ENG Western Blot kit (AWBK33068)

AWBK33068 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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  • Sensitivity:

ID4 Western Blot kit (AWBK38058)

AWBK38058 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
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  • Sensitivity:

EED Western Blot kit (AWBK38384)

AWBK38384 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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  • Sensitivity:

SSB Western Blot kit (AWBK40462)

AWBK40462 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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OTC Western Blot kit (AWBK41766)

AWBK41766 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

FST Western Blot kit (AWBK42287)

AWBK42287 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TMB Substrate, for Western Blot

F100029 1000 ml
EUR 992.4
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

TMB Substrate, for Western Blot

F129-100 100 ml
EUR 298.8
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

ERG Western Blot kit (AWBK100625)

AWBK100625 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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GLI2 Western Blot kit (AWBK31885)

AWBK31885 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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PAX4 Western Blot kit (AWBK32064)

AWBK32064 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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GLI1 Western Blot kit (AWBK32368)

AWBK32368 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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PAX7 Western Blot kit (AWBK32393)

AWBK32393 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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  • Sensitivity:

LHX6 Western Blot kit (AWBK32553)

AWBK32553 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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  • Sensitivity:

PAX7 Western Blot kit (AWBK32742)

AWBK32742 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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  • Sensitivity:

SOX5 Western Blot kit (AWBK33323)

AWBK33323 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

A1BG Western Blot kit (AWBK33810)

AWBK33810 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity: Human
  • Application:
  • Assay info:
  • Sensitivity:

NPM1 Western Blot kit (AWBK34094)

AWBK34094 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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  • Sensitivity:

LIG4 Western Blot kit (AWBK34122)

AWBK34122 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RNF2 Western Blot kit (AWBK34290)

AWBK34290 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ILF2 Western Blot kit (AWBK35731)

AWBK35731 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SPP1 Western Blot kit (AWBK36677)

AWBK36677 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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ZBP1 Western Blot kit (AWBK36711)

AWBK36711 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

G3BP Western Blot kit (AWBK37713)

AWBK37713 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

BRD7 Western Blot kit (AWBK39018)

AWBK39018 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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MSI2 Western Blot kit (AWBK41112)

AWBK41112 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TPM1 Western Blot kit (AWBK41392)

AWBK41392 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TSHR Western Blot kit (AWBK41870)

AWBK41870 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ASPN Western Blot kit (AWBK42487)

AWBK42487 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RORA Western Blot kit (AWBK45608)

AWBK45608 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PDCD4 Western Blot kit (AWBK09045)

AWBK09045 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ETS1 Western Blot kit (AWBK100604)

AWBK100604 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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GABRP Western Blot kit (AWBK13034)

AWBK13034 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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  • Sensitivity:

HTR1A Western Blot kit (AWBK13041)

AWBK13041 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RAB14 Western Blot kit (AWBK13107)

AWBK13107 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TNRC4 Western Blot kit (AWBK31525)

AWBK31525 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ESRRG Western Blot kit (AWBK31655)

AWBK31655 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

HIPK2 Western Blot kit (AWBK32586)

AWBK32586 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ZFP67 Western Blot kit (AWBK32749)

AWBK32749 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

OLIG2 Western Blot kit (AWBK32753)

AWBK32753 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SOX10 Western Blot kit (AWBK33326)

AWBK33326 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RPS16 Western Blot kit (AWBK33535)

AWBK33535 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ATP7A Western Blot kit (AWBK33797)

AWBK33797 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ZFP36 Western Blot kit (AWBK34385)

AWBK34385 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ARIH2 Western Blot kit (AWBK34418)

AWBK34418 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

KEAP1 Western Blot kit (AWBK34728)

AWBK34728 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

CLIC1 Western Blot kit (AWBK35045)

AWBK35045 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

KCNN2 Western Blot kit (AWBK35094)

AWBK35094 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

CLCN3 Western Blot kit (AWBK35504)

AWBK35504 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

P2RX7 Western Blot kit (AWBK35518)

AWBK35518 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TRPM3 Western Blot kit (AWBK35609)

AWBK35609 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PITX2 Western Blot kit (AWBK35634)

AWBK35634 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ZFP42 Western Blot kit (AWBK36244)

AWBK36244 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ANXA1 Western Blot kit (AWBK36568)

AWBK36568 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RUNX2 Western Blot kit (AWBK36678)

AWBK36678 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

Bapx1 Western Blot kit (AWBK36826)

AWBK36826 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SATB1 Western Blot kit (AWBK38208)

AWBK38208 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

JMJD3 Western Blot kit (AWBK40102)

AWBK40102 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

STAU1 Western Blot kit (AWBK40537)

AWBK40537 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PCBP1 Western Blot kit (AWBK40630)

AWBK40630 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

WNT2B Western Blot kit (AWBK41254)

AWBK41254 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

GNB1L Western Blot kit (AWBK41328)

AWBK41328 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

STK11 Western Blot kit (AWBK41743)

AWBK41743 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

KRT13 Western Blot kit (AWBK42031)

AWBK42031 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

IDH3A Western Blot kit (AWBK42237)

AWBK42237 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PBEF1 Western Blot kit (AWBK42255)

AWBK42255 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RSAD2 Western Blot kit (AWBK42696)

AWBK42696 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PANX2 Western Blot kit (AWBK42778)

AWBK42778 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PANX1 Western Blot kit (AWBK42783)

AWBK42783 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TGFBI Western Blot kit (AWBK44268)

AWBK44268 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PTPN2 Western Blot kit (AWBK45362)

AWBK45362 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TWIST1 Western Blot kit (AWBK37997)

AWBK37997 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TARDBP Western Blot kit (AWBK38941)

AWBK38941 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TARDBP Western Blot kit (AWBK38942)

AWBK38942 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SNRP70 Western Blot kit (AWBK40439)

AWBK40439 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

HNRPLL Western Blot kit (AWBK41102)

AWBK41102 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

HNRPA3 Western Blot kit (AWBK41195)

AWBK41195 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC1A5 Western Blot kit (AWBK42247)

AWBK42247 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ONECUT2 Western Blot kit (AWBK35750)

AWBK35750 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC12A1 Western Blot kit (AWBK41388)

AWBK41388 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

CEACAM6 Western Blot kit (AWBK41504)

AWBK41504 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC22A1 Western Blot kit (AWBK41516)

AWBK41516 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RHOBTB1 Western Blot kit (AWBK41883)

AWBK41883 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC22A7 Western Blot kit (AWBK42306)

AWBK42306 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

Interferon alpha Western Blot Antibody

20-abx137014
  • EUR 427.20
  • EUR 627.60
  • 0.5 mg
  • 1 mg

Colorimetric Western Blot Kit (OKRA00014)

OKRA00014 1 kit
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00015)

OKRA00015 1 each
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00016)

OKRA00016 1 kit
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00017)

OKRA00017 1 kit
EUR 730.8
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

TBS for Western Blot 10X, pH 7.4

42020384-1 1 L Ask for price

TBS for Western Blot 10X, pH 7.4

42020384-2 2 L Ask for price

TBS for Western Blot 10X, pH 7.4

42020384-3 4 L Ask for price

TBS for Western Blot 10X, pH 7.4

42020384-4 500 mL Ask for price

cyclin G2 Western Blot kit (AWBK03032)

AWBK03032 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ChonBlock Western Blot Buffer 1 (PBS)

9069P 10 ml
EUR 232.26
Description: ChonBlock Western Blot Buffer 1 (PBS)

ChonBlock Western Blot Buffer 1 (TBS)

9069T 10 ml
EUR 232.26
Description: ChonBlock Western Blot Buffer 1 (TBS)

Pure-IP Western Blot Detection Kit

PRB-5002 20 blots
EUR 470.4
Description: Western blot detection of IP proteins can exhibit high background due to the use of the same primary antibody in the IP and the Western blot. The Pure-IP Western Blot Detection Kit eliminates this background problem by using a proprietary HRP Conjugate for detection of the primary antibody.

Chemiluminescent Western Blot Kit (OKRA00001)

OKRA00001 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00002)

OKRA00002 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00003)

OKRA00003 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00004)

OKRA00004 1 kit
EUR 684
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

PBS Buffer Western Blot 10X, pH 7.4

41620013-1 1 L Ask for price

PBS Buffer Western Blot 10X, pH 7.4

41620013-2 2 L Ask for price

PBS Buffer Western Blot 10X, pH 7.4

41620013-3 4 L Ask for price

PBS Buffer Western Blot 10X, pH 7.4

41620013-4 6 L Ask for price

T-Pro Western Blot Stripping Reagent

JB11-K002 500ml/BT
EUR 213.6

AgileBlotter? Automated Western Blot Processor

ACT-ABH-40 each
EUR 20326.8

Nitrated Mol Wt Std for Western blot

NITT12-C 100 ul
EUR 343.2

Attoglow Western Blot Analysis Kit:Blocking Agent

K3171250-3 40 g
EUR 68

Western Blot Primary Antibody Diluent Buffer

abx090689-100ml 100 ml
EUR 218.4

Hypersensitive Western Blot Chemiluminescent Substrate

CR0001-10 20mL (sufficient reagents for 4000 cm2 of membrane)
EUR 200.4

Hypersensitive Western Blot Chemiluminescent Substrate

CR0001-25 50mL (sufficient reagents for 10000 cm2 of membrane)
EUR 376.8

Hypersensitive Western Blot Chemiluminescent Substrate

CR0001-5 10mL (sufficient reagents for 2000 cm2 of membrane)
EUR 139.2

TBS High Salt for Western Blot Washing 10X

10530026-1 1 L
EUR 40.37

TBS High Salt for Western Blot Washing 10X

10530026-2 4 L
EUR 111.42

Recombinant Human MutY Protein for Western blot

MUTY11-C 100 ul
EUR 343.2

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-050 500ml
EUR 144

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-100 2x500ml
EUR 176.4

ChonBlock Western Blot Buffer 2 for Goat (PBS)

90691P 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Goat (PBS)

ChonBlock Western Blot Buffer 2 for Goat (TBS)

90691T 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Goat (TBS)

Poly-ADP-ribosylated proteins for Western blot

ADPR11-C 100 ul
EUR 343.2

Rat Ceruloplasmin protein control for western blot

CP17-C 100 ul
EUR 343.2

Attoglow Western Blot Analysis Kit:Binding Buffer 20x

K3171250-2 120 ml
EUR 107

OPRB00208-10UG - mGPD (Western Blot Control) Protein

OPRB00208-10UG 10ug
EUR 256

OPRB00118-10UG - NGAL (Western Blot Control) Protein

OPRB00118-10UG 10ug
EUR 184

OPRB00121-10UG - M2PK (Western Blot Control) Protein

OPRB00121-10UG 10ug
EUR 246

SuperBrite? ECL Western Blot Substrate/Detection Kit

K824-50 each
EUR 229.2

ChonBlock Western Blot Buffer 2 for Rabbit (PBS)

90692P 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Rabbit (PBS)

ChonBlock Western Blot Buffer 2 for Rabbit (TBS)

90692T 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Rabbit (TBS)

Bovine DNAse 1 Protein Western blot +ve control

DNASE11-C 100 ul
EUR 343.2

Mouse Leptin protein Western blot +ve control # 1

LEP11-C 100 ul
EUR 343.2

Human Leptin protein Western blot +ve control # 2

LEP12-C 100 ul
EUR 343.2

Mouse Ceruloplasmin protein control for western blot

CP16-C 100 ul
EUR 343.2

OPRB00034-10UG - Rantes (Western Blot Control) Protein

OPRB00034-10UG 10ug
EUR 256

OPRB00037-10UG - Eotaxin (Western Blot Control) Protein

OPRB00037-10UG 10ug
EUR 184

OPRB00127-10UG - S100A10 (Western Blot Control) Protein

OPRB00127-10UG 10ug
EUR 184

OPRB00130-10UG - S100A11 (Western Blot Control) Protein

OPRB00130-10UG 10ug
EUR 184

Western blot recycling stripping buffer, 50 ml (10X)

90101 50 ml
EUR 285.6

Box Western Blot Polystyrene Transparent 95x30x16mm X5

DD62676 PK5
EUR 99.6

Attoglow Western Blot Analysis Kit: Millennium Enhancer

K3171250-1 100 ml
EUR 130

OPRB00067-10UG - Resistin (Western Blot Control) Protein

OPRB00067-10UG 10ug
EUR 246

OPRB00070-10UG - Visfatin (Western Blot Control) Protein

OPRB00070-10UG 10ug
EUR 184

Purified Human C1q protein Western blot +ve control

C1Q11-C 100 ul
EUR 343.2

HRP Western Blot Anti-IgG Antibody Kit (OKRA00006)

OKRA00006 100 ug
EUR 246
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

OPRB00139-10UG - Myoglobin (Western Blot Control) Protein

OPRB00139-10UG 10ug
EUR 184

Human C1 Esterase Inhibitor western blot +ve control

C1E11-C 100 ul
EUR 343.2

TBS Buffer Low Salt for Western Blot Washing 10X

10530027-1 1 L
EUR 32.46

TBS Buffer Low Salt for Western Blot Washing 10X

10530027-2 4 L
EUR 111.42

TBS Buffer Low Salt for Western Blot Washing 10X

10530027-3 500 mL
EUR 43.01

WesternOne? Marker Pen, HRP for Western blot chemiluminescent detection

ACT-WP each
EUR 172.8

NuGlo (luminol) ECL Substrate Kit for Western blot, 250 mls

80215-250 1 kit
EUR 196.8

Human Liver Ferritin protein Western blot +ve control

FERT12-C 100 ul
EUR 343.2

OPRB00254-10UG - ST-2 (Western Blot Control) Protein

OPRB00254-10UG 10ug
EUR 256

OPRB00274-10UG - Fractalkine (Western Blot Control) Protein

OPRB00274-10UG 10ug
EUR 256

Beta-Galactosidase (E. Coli) Protein for Western Blot

BGAL11-C 100 ul
EUR 343.2

Human Spleen Ferritin protein Western blot +ve control

FERT11-C 100 ul
EUR 343.2

Horse Spleen Ferritin protein Western blot +ve control

FERT13-C 100 ul
EUR 343.2

OPRB00251-10UG - ADAM 10 (Western Blot Control) Protein

OPRB00251-10UG 10ug
EUR 256

OPRB00076-10UG - MCP-1 (Western Blot Control) Protein

OPRB00076-10UG 10ug
EUR 246

Chicken Serum Albumin protein control for Western blot

ALBC15-C 100 ul
EUR 343.2

OPRB00031-10UG - ADAM 12S (Western Blot Control) Protein

OPRB00031-10UG 10ug
EUR 256

OPRB00052-10UG - Procalcitonin (Western Blot Control) Protein

OPRB00052-10UG 10ug
EUR 184

Purified Cholera Toxin protein control for Western Blot

CTOX11-C 100 ul
EUR 343.2
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