Combined bacterial and fungal targeted amplicon sequencing of respiratory samples: Does the DNA extraction method matter?

High-throughput sequencing techniques were used to analyze the diversity of respiratory microbiota in health and disease. Despite the extensive data available on the respiratory tract bacterial microbiota, the remnants of the less studied fungal components. This is partly due to the technical problems associated with fungal metagenomics analysis. In this study, we compared two protocols of DNA extraction and amplification of two targets for the combined fungal bacteria and fungi that are targeted amplicon sequencing analysis of sputum microbiota.Six breathing, selected randomly from a sample routine Mondor Hospital (Créteil, France) and treated anonymously, were tested after a routine culture of bacteria and fungi.

Two of spiked Fumigati Aspergillus and Aspergillus Nigri (105 conidia / ml). After the mechanical lysis, DNA was extracted using the automatic extraction of QIAsymphony® (AQE) or users PowerSoil® MoBio extraction (MPE). DNA yield and purity compared. DNA extracted from sputum spiked into the target (i) real-time PCR for detection of Aspergillus DNA and (ii) a combination of primary targeting barcode metagenomic analysis for ITS1 and ITS2 fungi, and bacteria V1-V2 and V3-V4 region of the 16S. amplicon libraries were prepared using reagents MiSeq V3 kit on the Illumina platform. Data were analyzed using software PyroMIC © and Shaman, and compared with culture results.AQE extraction provides a higher yield of the (ratio AQE / MPE DNA = 4.5 [1.3 to 11]) DNA in a shorter period of time.

Results of Aspergillus DNA is detected by qPCR were similar to sputum spiked regardless of the extraction protocol. extraction quite an impact on biodiversity or the relative abundance of bacterial communities using targeted amplicon sequencing (2/43 taxa impact). For fungi, the relative abundance of major taxa 4/11 affected and AQE results were closer to the culture results. V1-V2 or V3-V4 and ITS1 or ITS2 target is judged diversity major taxa of bacteria and fungi, but ITS2 and V3-V4 detected smaller taxa.Our results show the importance of joint extraction of DNA for bacteria and fungi targeted metagenomics respiratory samples. Extraction protocol may affect the results of the DNA and the relative abundance of a few bacteria, but more fungal taxa. For the analysis of fungi, ITS2 allow detection of a large number of taxa small compared with ITS1.

ACTG1 human mutation associated with high frequency hearing loss, and patients with mutations in this gene are good candidates for the electric acoustic stimulation. To better understand the genetic etiology of hearing loss cases, massively parallel DNA sequencing performed on a 7048 Japanese probands related hearing loss. Among the 1,336 patients with autosomal dominant hearing loss, we identified 15 probands (1.1%) with 13 ACTG1 potentially pathogenic variant. Six new variant and seven were reported previously.

We collect and analyze detailed clinical features of these patients. The average level of development of pure-tone hearing worsening of the average for the four frequency is 1.7 dB / year of age 0-50 years, and all individuals over 60 years old has a severe hearing loss. To better understand the mechanisms underlying causes of disease, the intracellular localization of the wild-type and mutant gamma-actins checked using NIH / 3T3 fibroblast cell line

Western Blot Enhancing Kit
K3172250 2500 cm2
EUR 188.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Interferon alpha Western Blot Antibody
20-abx137014
  • EUR 356.00
  • EUR 523.00
  • 0
  • 1
  • Shipped within 5-10 working days.

Hypersensitive Western Blot Chemiluminescent Substrate
CR0001-10 20mL (sufficient reagents for 4000 cm2 of membrane)
EUR 167.00

Hypersensitive Western Blot Chemiluminescent Substrate
CR0001-25 50mL (sufficient reagents for 10000 cm2 of membrane)
EUR 314.00

Hypersensitive Western Blot Chemiluminescent Substrate
CR0001-5 10mL (sufficient reagents for 2000 cm2 of membrane)
EUR 116.00

AgileBlotter? Automated Western Blot Processor
ACT-ABH-40
EUR 16939.00

TMB Substrate, for Western Blot
F100029 1000 ml
EUR 827.00
  • Product category: Buffer solution
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

TMB Substrate, for Western Blot
F129-100 100 ml
EUR 249.00
  • Product category: Buffer solution
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

PDCD4 Western Blot kit (AWBK09045)
AWBK09045 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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ETS1 Western Blot kit (AWBK100604)
AWBK100604 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ERG Western Blot kit (AWBK100625)
AWBK100625 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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GABRP Western Blot kit (AWBK13034)
AWBK13034 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
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HTR1A Western Blot kit (AWBK13041)
AWBK13041 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
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RAB14 Western Blot kit (AWBK13107)
AWBK13107 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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TNRC4 Western Blot kit (AWBK31525)
AWBK31525 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
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ESRRG Western Blot kit (AWBK31655)
AWBK31655 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
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GLI2 Western Blot kit (AWBK31885)
AWBK31885 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
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PAX4 Western Blot kit (AWBK32064)
AWBK32064 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
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AHR Western Blot kit (AWBK32243)
AWBK32243 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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GLI1 Western Blot kit (AWBK32368)
AWBK32368 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
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PAX7 Western Blot kit (AWBK32393)
AWBK32393 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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LHX6 Western Blot kit (AWBK32553)
AWBK32553 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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HIPK2 Western Blot kit (AWBK32586)
AWBK32586 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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PAX7 Western Blot kit (AWBK32742)
AWBK32742 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
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  • Assay info:
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ZFP67 Western Blot kit (AWBK32749)
AWBK32749 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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OLIG2 Western Blot kit (AWBK32753)
AWBK32753 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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ENG Western Blot kit (AWBK33068)
AWBK33068 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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SOX5 Western Blot kit (AWBK33323)
AWBK33323 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
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SOX10 Western Blot kit (AWBK33326)
AWBK33326 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
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RPS16 Western Blot kit (AWBK33535)
AWBK33535 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
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ATP7A Western Blot kit (AWBK33797)
AWBK33797 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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A1BG Western Blot kit (AWBK33810)
AWBK33810 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity: Human
  • Application:
  • Assay info:
  • Sensitivity:

NPM1 Western Blot kit (AWBK34094)
AWBK34094 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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LIG4 Western Blot kit (AWBK34122)
AWBK34122 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
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RNF2 Western Blot kit (AWBK34290)
AWBK34290 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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ZFP36 Western Blot kit (AWBK34385)
AWBK34385 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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ARIH2 Western Blot kit (AWBK34418)
AWBK34418 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

KEAP1 Western Blot kit (AWBK34728)
AWBK34728 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

CLIC1 Western Blot kit (AWBK35045)
AWBK35045 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

KCNN2 Western Blot kit (AWBK35094)
AWBK35094 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

CLCN3 Western Blot kit (AWBK35504)
AWBK35504 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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P2RX7 Western Blot kit (AWBK35518)
AWBK35518 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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TRPM3 Western Blot kit (AWBK35609)
AWBK35609 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PITX2 Western Blot kit (AWBK35634)
AWBK35634 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ILF2 Western Blot kit (AWBK35731)
AWBK35731 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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ONECUT2 Western Blot kit (AWBK35750)
AWBK35750 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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ZFP42 Western Blot kit (AWBK36244)
AWBK36244 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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ANXA1 Western Blot kit (AWBK36568)
AWBK36568 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
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  • Assay info:
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SPP1 Western Blot kit (AWBK36677)
AWBK36677 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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RUNX2 Western Blot kit (AWBK36678)
AWBK36678 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
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  • Assay info:
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ZBP1 Western Blot kit (AWBK36711)
AWBK36711 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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Bapx1 Western Blot kit (AWBK36826)
AWBK36826 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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G3BP Western Blot kit (AWBK37713)
AWBK37713 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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TWIST1 Western Blot kit (AWBK37997)
AWBK37997 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ID4 Western Blot kit (AWBK38058)
AWBK38058 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SATB1 Western Blot kit (AWBK38208)
AWBK38208 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

EED Western Blot kit (AWBK38384)
AWBK38384 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TARDBP Western Blot kit (AWBK38941)
AWBK38941 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TARDBP Western Blot kit (AWBK38942)
AWBK38942 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

BRD7 Western Blot kit (AWBK39018)
AWBK39018 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

JMJD3 Western Blot kit (AWBK40102)
AWBK40102 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SNRP70 Western Blot kit (AWBK40439)
AWBK40439 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SSB Western Blot kit (AWBK40462)
AWBK40462 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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STAU1 Western Blot kit (AWBK40537)
AWBK40537 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PCBP1 Western Blot kit (AWBK40630)
AWBK40630 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
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HNRPLL Western Blot kit (AWBK41102)
AWBK41102 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

MSI2 Western Blot kit (AWBK41112)
AWBK41112 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

HNRPA3 Western Blot kit (AWBK41195)
AWBK41195 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

WNT2B Western Blot kit (AWBK41254)
AWBK41254 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

GNB1L Western Blot kit (AWBK41328)
AWBK41328 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC12A1 Western Blot kit (AWBK41388)
AWBK41388 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TPM1 Western Blot kit (AWBK41392)
AWBK41392 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

CEACAM6 Western Blot kit (AWBK41504)
AWBK41504 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC22A1 Western Blot kit (AWBK41516)
AWBK41516 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

STK11 Western Blot kit (AWBK41743)
AWBK41743 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

OTC Western Blot kit (AWBK41766)
AWBK41766 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TSHR Western Blot kit (AWBK41870)
AWBK41870 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RHOBTB1 Western Blot kit (AWBK41883)
AWBK41883 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

KRT13 Western Blot kit (AWBK42031)
AWBK42031 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

IDH3A Western Blot kit (AWBK42237)
AWBK42237 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC1A5 Western Blot kit (AWBK42247)
AWBK42247 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PBEF1 Western Blot kit (AWBK42255)
AWBK42255 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

FST Western Blot kit (AWBK42287)
AWBK42287 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SLC22A7 Western Blot kit (AWBK42306)
AWBK42306 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ASPN Western Blot kit (AWBK42487)
AWBK42487 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RSAD2 Western Blot kit (AWBK42696)
AWBK42696 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PANX2 Western Blot kit (AWBK42778)
AWBK42778 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PANX1 Western Blot kit (AWBK42783)
AWBK42783 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

TGFBI Western Blot kit (AWBK44268)
AWBK44268 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PTPN2 Western Blot kit (AWBK45362)
AWBK45362 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RORA Western Blot kit (AWBK45608)
AWBK45608 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00001)
OKRA00001 1 kit
EUR 570.00
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00002)
OKRA00002 1 kit
EUR 570.00
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00003)
OKRA00003 1 kit
EUR 570.00
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00004)
OKRA00004 1 kit
EUR 570.00
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00014)
OKRA00014 1 kit
EUR 609.00
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00015)
OKRA00015 1 each
EUR 609.00
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00016)
OKRA00016 1 kit
EUR 609.00
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00017)
OKRA00017 1 kit
EUR 609.00
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

ChonBlock Western Blot Buffer 1 (PBS)
9069P 10 ml
EUR 193.55
Description: ChonBlock Western Blot Buffer 1 (PBS)

ChonBlock Western Blot Buffer 1 (TBS)
9069T 10 ml
EUR 193.55
Description: ChonBlock Western Blot Buffer 1 (TBS)

Western Blot Primary Antibody Diluent Buffer
abx090689-100ml 100 ml
EUR 182.00
  • Shipped within 5-10 working days.

Attoglow Western Blot Analysis Kit:Blocking Agent
K3171250-3 40 g
EUR 109.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

T-Pro Western Blot Stripping Reagent
JB11-K002 500ml/BT
EUR 178.00

Pure-IP Western Blot Detection Kit
PRB-5002 20 blots
EUR 392.00
Description: Western blot detection of IP proteins can exhibit high background due to the use of the same primary antibody in the IP and the Western blot. The Pure-IP Western Blot Detection Kit eliminates this background problem by using a proprietary HRP Conjugate for detection of the primary antibody.

cyclin G2 Western Blot kit (AWBK03032)
AWBK03032 10 reactions
EUR 647.00
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

Poly-ADP-ribosylated proteins for Western blot
ADPR11-C 100 ul
EUR 286.00

Mouse Ceruloplasmin protein control for western blot
CP16-C 100 ul
EUR 286.00

Rat Ceruloplasmin protein control for western blot
CP17-C 100 ul
EUR 286.00

SuperBrite? ECL Western Blot Substrate/Detection Kit
K824-50
EUR 191.00

Attoglow Western Blot Analysis Kit: Millennium Enhancer
K3171250-1 100 ml
EUR 163.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Attoglow Western Blot Analysis Kit:Binding Buffer 20x
K3171250-2 120 ml
EUR 143.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Nitrated Mol Wt Std for Western blot
NITT12-C 100 ul
EUR 286.00

Recombinant Human MutY Protein for Western blot
MUTY11-C 100 ul
EUR 286.00

Bovine DNAse 1 Protein Western blot +ve control
DNASE11-C 100 ul
EUR 286.00

Purified Cholera Toxin protein control for Western Blot
CTOX11-C 100 ul
EUR 286.00

Western blot recycling stripping buffer, 50 ml (10X)
90101 50 ml
EUR 238.00

ChonBlock Western Blot Buffer 2 for Goat (PBS)
90691P 10 ml
EUR 121.05
Description: ChonBlock Western Blot Buffer 2 for Goat (PBS)

ChonBlock Western Blot Buffer 2 for Goat (TBS)
90691T 10 ml
EUR 121.05
Description: ChonBlock Western Blot Buffer 2 for Goat (TBS)

ChonBlock Western Blot Buffer 2 for Rabbit (PBS)
90692P 10 ml
EUR 121.05
Description: ChonBlock Western Blot Buffer 2 for Rabbit (PBS)

ChonBlock Western Blot Buffer 2 for Rabbit (TBS)
90692T 10 ml
EUR 121.05
Description: ChonBlock Western Blot Buffer 2 for Rabbit (TBS)

Human C1 Esterase Inhibitor western blot +ve control
C1E11-C 100 ul
EUR 286.00

Purified Human C1q protein Western blot +ve control
C1Q11-C 100 ul
EUR 286.00

Beta-Galactosidase (E. Coli) Protein for Western Blot
BGAL11-C 100 ul
EUR 286.00

Recombinant Beta Lactamase protein control for Western blot
BLAC11-C 100 ul
EUR 286.00

Chicken Serum Albumin protein control for Western blot
ALBC15-C 100 ul
EUR 286.00

Human Spleen Ferritin protein Western blot +ve control
FERT11-C 100 ul
EUR 286.00

Human Liver Ferritin protein Western blot +ve control
FERT12-C 100 ul
EUR 286.00

Horse Spleen Ferritin protein Western blot +ve control
FERT13-C 100 ul
EUR 286.00

Mouse Leptin protein Western blot +ve control # 1
LEP11-C 100 ul
EUR 286.00

Human Leptin protein Western blot +ve control # 2
LEP12-C 100 ul
EUR 286.00

Human Pancreatic Amylase protein control for Western blot
PAMY13-C 100 ul
EUR 286.00

HRP Western Blot Anti-IgG Antibody Kit (OKRA00006)
OKRA00006 100 ug
EUR 205.00
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Human Liver Cathepsin B protein control for Western blot
CTHB11-C 100 ul
EUR 286.00

Human Liver Cathepsin D protein control for Western blot
CTHD11-C 100 ul
EUR 286.00

Human Liver Cathepsin G protein control for Western blot
CTHG11-C 100 ul
EUR 286.00

Human Liver Cathepsin H protein control for Western blot
CTHH11-C 100 ul
EUR 286.00

Human Liver Cathepsin L protein control for Western blot
CTHL11-C 100 ul
EUR 286.00

Single solution TMB substrate (membrane) solution for western blot
80110 100 mls
EUR 225.00

Western blot Kit for Rabbit Primary Antibodies, Chemilum. Substrate
80200-Rb 1 kit
EUR 651.00

Western blot Kit for Goat Primary Antibodies, Chemilum. Substrate
80201-Gt 1 kit
EUR 651.00

Western blot Kit for Mouse Primary Antibodies, Chemilum. Substrate
80202-Mo 1 kit
EUR 651.00

Western blot Kit for Rat Primary Antibodies, Chemilum. Substrate
80203-Rt 1 kit
EUR 651.00

Western blot Kit for Chicken Primary Antibodies, Chemilum. Substrate
80204-Ch 1 kit
EUR 651.00

Western blot Kit for Monkey Primary Antibodies, Chemilum. Substrate
80205-Mk 1 kit
EUR 651.00

Western blot Kit for Human Primary Antibodies, Chemilum. Substrate
80207-Hu 1 kit
EUR 651.00

Western blot Kit for Sheep Primary Antibodies; Chemilum. Substrate
80208-Sh 1 kit
EUR 651.00

Western blot Kit for Pig Primary Antibodies; Chemilum. Substrate
80209-Pg 1 kit
EUR 651.00

NuGlo ECL Substrate Kit for Western blot, 400 mls
80215 1 kit
EUR 408.00

Camelpox virus H3L/p35 protein control for western blot
CPOX11-C 100 ul
EUR 286.00

Human Adipsin/Factor D protein Western blot +ve control
ADN12-C 100 ul
EUR 286.00

WesternOne? Marker Pen, HRP for Western blot chemiluminescent detection
ACT-WP
EUR 144.00

Recombinant human BAFF-R protein control for Western blot
BAFFR11-C 100 ul
EUR 286.00

Human recombinant, purified BMP2 protein Western blot +ve control
BMP21-C 100 ul
EUR 286.00

Human recombinant, purified BMP3 protein Western blot +ve control
BMP31-C 100 ul
EUR 286.00

Human recombinant, purified BMP4 protein Western blot +ve control
BMP41-C 100 ul
EUR 286.00

Human recombinant, purified BMP5 protein Western blot +ve control
BMP51-C 100 ul
EUR 286.00

Human recombinant, purified BMP6 protein Western blot +ve control
BMP61-C 100 ul
EUR 286.00

Purified Mouse serum albumin protein control for Western blot
ALB11-C 100 ul
EUR 286.00

Purified Human serum albumin protein control for Western blot
ALBH13-C 100 ul
EUR 286.00

Purified rat serum albumin protein control for Western blot
ALBR12-C 100 ul
EUR 286.00

Purified Sheep serum albumin protein control for Western blot
ALBSH20-C 100 ul
EUR 286.00

Purified Ram Cyclooxygenase (Cox-1) Western blot +ve control
COX11-C 100 ul
EUR 286.00

Purified Ram Cyclooxygenase (Cox-2) Western blot +ve control
COX21-C 100 ul
EUR 286.00

Human hemoglobin (non-glycated) protein control for western blot
HEMG13-C 100 ul
EUR 286.00

Recombinant Mycoplasma Pulmonis (Mp) protein control for Western Blot
MPUL11-C 100 ul
EUR 445.00
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