Combined use of single molecule real-time DNA sequencing technology and culture-dependent methods to analyze the functional microorganisms in inoculated raw wheat Qu.

Inoculated raw wheat Qu (IRWQ), which can be produced with high efficiency and low cost while maintaining stable quality, is the new Qu who have applied for huangjiu brewing. In this study, a single molecule real-time DNA sequencing technology and culture dependent method combined for the first time to study the microbiota and function of microorganisms principle in IRWQ. The glucoamylase, amylase and protease IRWQ contents are 1.17, 1.55 and 2.87 times greater, respectively, than traditional wheat Qu. As traditional wheat Qu, the main volatile flavor compounds in IRWQ is alcohol; However, the ester content is much higher and much lower acid content. Real-time single molecule DNA sequencing technology identified 18 species of fungi and 59 species of bacteria in IRWQ. Then, 30 species were isolated by culture dependent method.

These species represent approximately 80% of total fungal microbiota and 35% of the total bacterial microbiota. Mold is a major contributor to the activities of glucoamylase, amylase and protease. Aspergillus flavus has the highest glucoamylase and protease activity. Bacillus subtilis and Bacillus amyloliquefaciens also produce high hydrolytic enzyme activity. Saccharomyces cerevisiae produces the largest amount of aromatic compounds, alcohols, esters and acids. Aldehydes and ketones were mainly produced by molds.

Sequencing by synthesis is a significant method for high-throughput DNA sequencing. Here, we synthesized terminal-induced-emission collected luminogen (AIEgen) labeled nucleotides (dNTP-HCAP) that can serve as a substrate for a polymerase and is applied to the sequencing of small DNA fragments. In the process of DNA amplification, ratiometric AIEgens released from dNTP-HCAP and aggregates through the effect of phosphatase, which produces a change in the ratiometric fluorescent signal.

With AIEgen-labeled nucleotides, we accomplished a small DNA fragment sequences through a double change in fluorescence. In addition, we achieved a single nucleotide polymorphism differentiation through rolling circle amplification reaction without the addition of a signal probe, rapid and cost-effective. The introduction of DNA synthesis AIEgens ratiometric to make the detection of a DNA sequence that is more efficient and accurate. Therefore, the development of AIEgen-labeled nucleotides meaningful for the study of DNA sequencing method.

D6 Antibody Western Blot Positive Control
MBS542503-5x5Applications	MyBiosource	5x5Applications	EUR 1350

DCC Antibody Western Blot Positive Control
MBS542700-5Applications	MyBiosource	5Applications	EUR 335

DCC Antibody Western Blot Positive Control
MBS542700-5x5Applications	MyBiosource	5x5Applications	EUR 1350

DCK Antibody Western Blot Positive Control
MBS542701-5Applications	MyBiosource	5Applications	EUR 335

DCK Antibody Western Blot Positive Control
MBS542701-5x5Applications	MyBiosource	5x5Applications	EUR 1350

HE4 Antibody Western Blot Positive Control
MBS542751-5Applications	MyBiosource	5Applications	EUR 335

HE4 Antibody Western Blot Positive Control
MBS542751-5x5Applications	MyBiosource	5x5Applications	EUR 1350

FGR Antibody Western Blot Positive Control
MBS542768-5Applications	MyBiosource	5Applications	EUR 335

FGR Antibody Western Blot Positive Control
MBS542768-5x5Applications	MyBiosource	5x5Applications	EUR 1350

FSH Antibody Western Blot Positive Control
MBS542774-5Applications	MyBiosource	5Applications	EUR 335

FSH Antibody Western Blot Positive Control
MBS542774-5x5Applications	MyBiosource	5x5Applications	EUR 1350

FYN Antibody Western Blot Positive Control
MBS542779-5Applications	MyBiosource	5Applications	EUR 335

FYN Antibody Western Blot Positive Control
MBS542779-5x5Applications	MyBiosource	5x5Applications	EUR 1350

CMT Antibody Western Blot Positive Control
MBS542857-5Applications	MyBiosource	5Applications	EUR 335

CMT Antibody Western Blot Positive Control
MBS542857-5x5Applications	MyBiosource	5x5Applications	EUR 1350

ILK Antibody Western Blot Positive Control
MBS542871-5Applications	MyBiosource	5Applications	EUR 335

ILK Antibody Western Blot Positive Control
MBS542871-5x5Applications	MyBiosource	5x5Applications	EUR 1350

ITK Antibody Western Blot Positive Control
MBS542882-5Applications	MyBiosource	5Applications	EUR 335

ITK Antibody Western Blot Positive Control
MBS542882-5x5Applications	MyBiosource	5x5Applications	EUR 1350

LAT Antibody Western Blot Positive Control
MBS542910-5Applications	MyBiosource	5Applications	EUR 335

LAT Antibody Western Blot Positive Control
MBS542910-5x5Applications	MyBiosource	5x5Applications	EUR 1350

p63 Antibody Western Blot Positive Control
MBS543019-5Applications	MyBiosource	5Applications	EUR 335

p63 Antibody Western Blot Positive Control
MBS543019-5x5Applications	MyBiosource	5x5Applications	EUR 1350

MSF Antibody Western Blot Positive Control
MBS543234-5Applications	MyBiosource	5Applications	EUR 335

MSF Antibody Western Blot Positive Control
MBS543234-5x5Applications	MyBiosource	5x5Applications	EUR 1350

trp Antibody Western Blot Positive Control
MBS543354-5Applications	MyBiosource	5Applications	EUR 335

trp Antibody Western Blot Positive Control
MBS543354-5x5Applications	MyBiosource	5x5Applications	EUR 1350

CCR1 Antibody Western Blot Positive Control
MBS542604-5Applications	MyBiosource	5Applications	EUR 335

CCR1 Antibody Western Blot Positive Control
MBS542604-5x5Applications	MyBiosource	5x5Applications	EUR 1350

CCR2 Antibody Western Blot Positive Control
MBS542605-5Applications	MyBiosource	5Applications	EUR 335

CCR2 Antibody Western Blot Positive Control
MBS542605-5x5Applications	MyBiosource	5x5Applications	EUR 1350

CD36 Antibody Western Blot Positive Control
MBS542618-5Applications	MyBiosource	5Applications	EUR 335

CD36 Antibody Western Blot Positive Control
MBS542618-5x5Applications	MyBiosource	5x5Applications	EUR 1350

CD80 Antibody Western Blot Positive Control
MBS542622-5Applications	MyBiosource	5Applications	EUR 335

CD80 Antibody Western Blot Positive Control
MBS542622-5x5Applications	MyBiosource	5x5Applications	EUR 1350

DDX6 Antibody Western Blot Positive Control
MBS542702-5Applications	MyBiosource	5Applications	EUR 335

DDX6 Antibody Western Blot Positive Control
MBS542702-5x5Applications	MyBiosource	5x5Applications	EUR 1350

JunD Antibody Western Blot Positive Control
MBS542708-5Applications	MyBiosource	5Applications	EUR 335

JunD Antibody Western Blot Positive Control
MBS542708-5x5Applications	MyBiosource	5x5Applications	EUR 1350

E2F2 Antibody Western Blot Positive Control
MBS542718-5Applications	MyBiosource	5Applications	EUR 335

E2F2 Antibody Western Blot Positive Control
MBS542718-5x5Applications	MyBiosource	5x5Applications	EUR 1350

E2F3 Antibody Western Blot Positive Control
MBS542719-5Applications	MyBiosource	5Applications	EUR 335

E2F3 Antibody Western Blot Positive Control
MBS542719-5x5Applications	MyBiosource	5x5Applications	EUR 1350

E2F4 Antibody Western Blot Positive Control
MBS542720-5Applications	MyBiosource	5Applications	EUR 335

E2F4 Antibody Western Blot Positive Control
MBS542720-5x5Applications	MyBiosource	5x5Applications	EUR 1350

E2F5 Antibody Western Blot Positive Control
MBS542721-5Applications	MyBiosource	5Applications	EUR 335

E2F5 Antibody Western Blot Positive Control
MBS542721-5x5Applications	MyBiosource	5x5Applications	EUR 1350

EHD1 Antibody Western Blot Positive Control
MBS542725-5Applications	MyBiosource	5Applications	EUR 335

EHD1 Antibody Western Blot Positive Control
MBS542725-5x5Applications	MyBiosource	5x5Applications	EUR 1350

EHD2 Antibody Western Blot Positive Control
MBS542726-5Applications	MyBiosource	5Applications	EUR 335

EHD2 Antibody Western Blot Positive Control
MBS542726-5x5Applications	MyBiosource	5x5Applications	EUR 1350

EHD3 Antibody Western Blot Positive Control
MBS542727-5Applications	MyBiosource	5Applications	EUR 335

EHD3 Antibody Western Blot Positive Control
MBS542727-5x5Applications	MyBiosource	5x5Applications	EUR 1350

GGCX Antibody Western Blot Positive Control
MBS542797-5Applications	MyBiosource	5Applications	EUR 335

GGCX Antibody Western Blot Positive Control
MBS542797-5x5Applications	MyBiosource	5x5Applications	EUR 1350

hopQ Antibody Western Blot Positive Control
MBS542851-5Applications	MyBiosource	5Applications	EUR 335

hopQ Antibody Western Blot Positive Control
MBS542851-5x5Applications	MyBiosource	5x5Applications	EUR 1350

MIG7 Antibody Western Blot Positive Control
MBS542954-5Applications	MyBiosource	5Applications	EUR 335

MIG7 Antibody Western Blot Positive Control
MBS542954-5x5Applications	MyBiosource	5x5Applications	EUR 1350

MSH4 Antibody Western Blot Positive Control
MBS542964-5Applications	MyBiosource	5Applications	EUR 335

MSH4 Antibody Western Blot Positive Control
MBS542964-5x5Applications	MyBiosource	5x5Applications	EUR 1350

MSX1 Antibody Western Blot Positive Control
MBS542965-5Applications	MyBiosource	5Applications	EUR 335

MSX1 Antibody Western Blot Positive Control
MBS542965-5x5Applications	MyBiosource	5x5Applications	EUR 1350

mTOR Antibody Western Blot Positive Control
MBS542966-5Applications	MyBiosource	5Applications	EUR 335

mTOR Antibody Western Blot Positive Control
MBS542966-5x5Applications	MyBiosource	5x5Applications	EUR 1350

MUC1 Antibody Western Blot Positive Control
MBS542967-5Applications	MyBiosource	5Applications	EUR 335

MUC1 Antibody Western Blot Positive Control
MBS542967-5x5Applications	MyBiosource	5x5Applications	EUR 1350

NTH1 Antibody Western Blot Positive Control
MBS543003-5Applications	MyBiosource	5Applications	EUR 335

NTH1 Antibody Western Blot Positive Control
MBS543003-5x5Applications	MyBiosource	5x5Applications	EUR 1350

OSR2 Antibody Western Blot Positive Control
MBS543012-5Applications	MyBiosource	5Applications	EUR 335

OSR2 Antibody Western Blot Positive Control
MBS543012-5x5Applications	MyBiosource	5x5Applications	EUR 1350

P2Y1 Antibody Western Blot Positive Control
MBS543016-5Applications	MyBiosource	5Applications	EUR 335

P2Y1 Antibody Western Blot Positive Control
MBS543016-5x5Applications	MyBiosource	5x5Applications	EUR 1350

PELO Antibody Western Blot Positive Control
MBS543106-5Applications	MyBiosource	5Applications	EUR 335

PELO Antibody Western Blot Positive Control
MBS543106-5x5Applications	MyBiosource	5x5Applications	EUR 1350

Pin1 Antibody Western Blot Positive Control
MBS543125-5Applications	MyBiosource	5Applications	EUR 335

Pin1 Antibody Western Blot Positive Control
MBS543125-5x5Applications	MyBiosource	5x5Applications	EUR 1350

POP4 Antibody Western Blot Positive Control
MBS543148-5Applications	MyBiosource	5Applications	EUR 335

POP4 Antibody Western Blot Positive Control
MBS543148-5x5Applications	MyBiosource	5x5Applications	EUR 1350

PTIP Antibody Western Blot Positive Control
MBS543178-5Applications	MyBiosource	5Applications	EUR 350

PTIP Antibody Western Blot Positive Control
MBS543178-5x5Applications	MyBiosource	5x5Applications	EUR 1435

RCE1 Antibody Western Blot Positive Control
MBS543190-5Applications	MyBiosource	5Applications	EUR 335

RCE1 Antibody Western Blot Positive Control
MBS543190-5x5Applications	MyBiosource	5x5Applications	EUR 1350

RRF3 Antibody Western Blot Positive Control
MBS543191-5Applications	MyBiosource	5Applications	EUR 335

RRF3 Antibody Western Blot Positive Control
MBS543191-5x5Applications	MyBiosource	5x5Applications	EUR 1350

ROR2 Antibody Western Blot Positive Control
MBS543209-5Applications	MyBiosource	5Applications	EUR 335

ROR2 Antibody Western Blot Positive Control
MBS543209-5x5Applications	MyBiosource	5x5Applications	EUR 1350

SARA Antibody Western Blot Positive Control
MBS543219-5Applications	MyBiosource	5Applications	EUR 335

SARA Antibody Western Blot Positive Control
MBS543219-5x5Applications	MyBiosource	5x5Applications	EUR 1350

ARTS Antibody Western Blot Positive Control
MBS543229-5Applications	MyBiosource	5Applications	EUR 335

ARTS Antibody Western Blot Positive Control
MBS543229-5x5Applications	MyBiosource	5x5Applications	EUR 1350

SHQ1 Antibody Western Blot Positive Control
MBS543255-5Applications	MyBiosource	5Applications	EUR 335

SHQ1 Antibody Western Blot Positive Control
MBS543255-5x5Applications	MyBiosource	5x5Applications	EUR 1350

TGM1 Antibody Western Blot Positive Control
MBS543308-5Applications	MyBiosource	5Applications	EUR 335

TGM1 Antibody Western Blot Positive Control
MBS543308-5x5Applications	MyBiosource	5x5Applications	EUR 1350

TIA1 Antibody Western Blot Positive Control
MBS543310-5Applications	MyBiosource	5Applications	EUR 335

TIA1 Antibody Western Blot Positive Control
MBS543310-5x5Applications	MyBiosource	5x5Applications	EUR 1350

TLR4 Antibody Western Blot Positive Control
MBS543323-5Applications	MyBiosource	5Applications	EUR 335

TLR4 Antibody Western Blot Positive Control
MBS543323-5x5Applications	MyBiosource	5x5Applications	EUR 1350

TLR7 Antibody Western Blot Positive Control
MBS543326-5Applications	MyBiosource	5Applications	EUR 335

TLR7 Antibody Western Blot Positive Control
MBS543326-5x5Applications	MyBiosource	5x5Applications	EUR 1350

TRIF Antibody Western Blot Positive Control
MBS543351-5Applications	MyBiosource	5Applications	EUR 335

TRIF Antibody Western Blot Positive Control
MBS543351-5x5Applications	MyBiosource	5x5Applications	EUR 1350

UBR1 Antibody Western Blot Positive Control
MBS543363-5Applications	MyBiosource	5Applications	EUR 335

UBR1 Antibody Western Blot Positive Control
MBS543363-5x5Applications	MyBiosource	5x5Applications	EUR 1350

UCN2 Antibody Western Blot Positive Control
MBS543364-5Applications	MyBiosource	5Applications	EUR 335

UCN2 Antibody Western Blot Positive Control
MBS543364-5x5Applications	MyBiosource	5x5Applications	EUR 1350

ULK2 Antibody Western Blot Positive Control
MBS543367-5Applications	MyBiosource	5Applications	EUR 335

ULK2 Antibody Western Blot Positive Control
MBS543367-5x5Applications	MyBiosource	5x5Applications	EUR 1350

ULK4 Antibody Western Blot Positive Control
MBS543369-5Applications	MyBiosource	5Applications	EUR 335

ULK4 Antibody Western Blot Positive Control
MBS543369-5x5Applications	MyBiosource	5x5Applications	EUR 1350

YES1 Antibody Western Blot Positive Control
MBS543404-5Applications	MyBiosource	5Applications	EUR 335

YES1 Antibody Western Blot Positive Control
MBS543404-5x5Applications	MyBiosource	5x5Applications	EUR 1350

ACK1 Antibody Western Blot Positive Control
MBS542502-5Applications	MyBiosource	5Applications	EUR 335

ACK1 Antibody Western Blot Positive Control
MBS542502-5x5Applications	MyBiosource	5x5Applications	EUR 1350

ARF4 Antibody Western Blot Positive Control
MBS542545-5Applications	MyBiosource	5Applications	EUR 335

ARF4 Antibody Western Blot Positive Control
MBS542545-5x5Applications	MyBiosource	5x5Applications	EUR 1350

Western Blot Primary Antibody Diluent Buffer
abx090689-100l	Abbexa	100 µl	EUR 125

Western Blot Primary Antibody Diluent Buffer
abx090689-100ml	Abbexa	100 ml	EUR 218.4

Western Blot Primary Antibody Diluent Buffer
abx090689-1ml	Abbexa	1 ml	Ask for price

Western Blot Primary Antibody Diluent Buffer
abx090689-200l	Abbexa	200 µl	Ask for price

NT5C2 Antibody Western Blot Positive Control
MBS542644-5Applications	MyBiosource	5Applications	EUR 335

NT5C2 Antibody Western Blot Positive Control
MBS542644-5x5Applications	MyBiosource	5x5Applications	EUR 1350

CXCR3 Antibody Western Blot Positive Control
MBS542665-5Applications	MyBiosource	5Applications	EUR 335

CXCR3 Antibody Western Blot Positive Control
MBS542665-5x5Applications	MyBiosource	5x5Applications	EUR 1350

CXCR5 Antibody Western Blot Positive Control
MBS542667-5Applications	MyBiosource	5Applications	EUR 335

CXCR5 Antibody Western Blot Positive Control
MBS542667-5x5Applications	MyBiosource	5x5Applications	EUR 1350

CXCR6 Antibody Western Blot Positive Control
MBS542668-5Applications	MyBiosource	5Applications	EUR 335

CXCR6 Antibody Western Blot Positive Control
MBS542668-5x5Applications	MyBiosource	5x5Applications	EUR 1350

DISC1 Antibody Western Blot Positive Control
MBS542707-5Applications	MyBiosource	5Applications	EUR 335

DISC1 Antibody Western Blot Positive Control
MBS542707-5x5Applications	MyBiosource	5x5Applications	EUR 1350

DOC2B Antibody Western Blot Positive Control
MBS542710-5Applications	MyBiosource	5Applications	EUR 335

DOC2B Antibody Western Blot Positive Control
MBS542710-5x5Applications	MyBiosource	5x5Applications	EUR 1350

ergo1 Antibody Western Blot Positive Control
MBS542750-5Applications	MyBiosource	5Applications	EUR 335

ergo1 Antibody Western Blot Positive Control
MBS542750-5x5Applications	MyBiosource	5x5Applications	EUR 1350

FUBP1 Antibody Western Blot Positive Control
MBS542776-5Applications	MyBiosource	5Applications	EUR 335

FUBP1 Antibody Western Blot Positive Control
MBS542776-5x5Applications	MyBiosource	5x5Applications	EUR 1350

DDIT3 Antibody Western Blot Positive Control
MBS542785-5Applications	MyBiosource	5Applications	EUR 335

DDIT3 Antibody Western Blot Positive Control
MBS542785-5x5Applications	MyBiosource	5x5Applications	EUR 1350

GIRK1 Antibody Western Blot Positive Control
MBS542799-5Applications	MyBiosource	5Applications	EUR 335

GIRK1 Antibody Western Blot Positive Control
MBS542799-5x5Applications	MyBiosource	5x5Applications	EUR 1350

GIRK2 Antibody Western Blot Positive Control
MBS542800-5Applications	MyBiosource	5Applications	EUR 335

GIRK2 Antibody Western Blot Positive Control
MBS542800-5x5Applications	MyBiosource	5x5Applications	EUR 1350

GIRK4 Antibody Western Blot Positive Control
MBS542801-5Applications	MyBiosource	5Applications	EUR 335

GIRK4 Antibody Western Blot Positive Control
MBS542801-5x5Applications	MyBiosource	5x5Applications	EUR 1350

GIRK4 Antibody Western Blot Positive Control
MBS542802-5Applications	MyBiosource	5Applications	EUR 335

GIRK4 Antibody Western Blot Positive Control
MBS542802-5x5Applications	MyBiosource	5x5Applications	EUR 1350

GLP2R Antibody Western Blot Positive Control
MBS542803-5Applications	MyBiosource	5Applications	EUR 335

GLP2R Antibody Western Blot Positive Control
MBS542803-5x5Applications	MyBiosource	5x5Applications	EUR 1350

GRIK1 Antibody Western Blot Positive Control
MBS542806-5Applications	MyBiosource	5Applications	EUR 335

GRIK1 Antibody Western Blot Positive Control
MBS542806-5x5Applications	MyBiosource	5x5Applications	EUR 1350

IFI16 Antibody Western Blot Positive Control
MBS542860-5Applications	MyBiosource	5Applications	EUR 335

IFI16 Antibody Western Blot Positive Control
MBS542860-5x5Applications	MyBiosource	5x5Applications	EUR 1350

IL17B Antibody Western Blot Positive Control
MBS542866-5Applications	MyBiosource	5Applications	EUR 335

IL17B Antibody Western Blot Positive Control
MBS542866-5x5Applications	MyBiosource	5x5Applications	EUR 1350

Inp2p Antibody Western Blot Positive Control
MBS542875-5Applications	MyBiosource	5Applications	EUR 335

Inp2p Antibody Western Blot Positive Control
MBS542875-5x5Applications	MyBiosource	5x5Applications	EUR 1350

FNDC5 Antibody Western Blot Positive Control
MBS542880-5Applications	MyBiosource	5Applications	EUR 335

FNDC5 Antibody Western Blot Positive Control
MBS542880-5x5Applications	MyBiosource	5x5Applications	EUR 1350

LAMP1 Antibody Western Blot Positive Control
MBS542907-5Applications	MyBiosource	5Applications	EUR 335

LAMP1 Antibody Western Blot Positive Control
MBS542907-5x5Applications	MyBiosource	5x5Applications	EUR 1350

LARP7 Antibody Western Blot Positive Control
MBS542909-5Applications	MyBiosource	5Applications	EUR 335